PRODUCTION OF INTERSPECIFIC HYBRIDS BETWEEN ALSTROEMERIA-LIGTU L HYBRID AND ALSTROEMERIA-PELEGRINA L VAR ROSEA BY OVULE CULTURE

Interspecific hybrids between Alstroemeria ligtu L. hybrid and A. pele grina L. var, rosea were produced by using ovule culture. In this cros s combination, viable seeds could not be obtained in the reciprocal cr ossings. Histological observation revealed that the hybrid embryos abo rted around 28 days after pollination in the crossing of A, ligtu hybr id x A, pelegrina var. rosea and around 35 days after pollination in t he reverse crossing without appreciable development of endosperm, When ovules with placenta were transferred to 2 g/l Gelrite-solidified MS medium containing 3 % (w/v) sucrose before the embryo degeneration, pl antlets were formed in the reciprocal cross combinations, However matu re plants were obtained only in a cross of A, ligtu hybrid x A, pelegr ina var, rosea, The hybrid plants had intermediate characteristics bet ween the parents, and complete haploid genomes of the both parents. Hy bridity of the plants was also confirmed by DNA analysis.