Fluorescence, in situ hybridization with chromosome paint probes: A novel approach to assess aneuploidy in human sperm nuclei

Purpose: Fluorescence in situ hybridization (FISH) using whole-chromosome p aint probes was performed to evaluate disomy and diploidy frequency for chr omosomes 1, 18, 19, and 22 in human sperm nuclei. Methods: Ten subjects of proven fertility and normal spermatic parameters w ere included in tilt study: A dual-color FISH method was carried out. Results: A total of 157,896 spermatozoa was scored. The mean frequencies of disomic sperm for chromosomes 1, 18, 19, and 22 Mere 0.22% (range, 0.19 to 0.28%), 0.24% (range, 0.14 to 0.37%), 0.22% (range, 0.17 to 0.30%), and 0. 25% (range, 0.21 to 0.29%), respectively: The mean frequency of diploidy wa s 0.14% (range, 0.09 to 0.18%). No interindividual and interchromosomal var iations in the aneuploidy frequency were observed between the different sub jects. Conclusions: FISH with whole-chromosome paint probes provides a novel and e fficient approach for disomy assessment in human sperm nuclei.