QUANTITATIVE-EVALUATION OF THE IL-1-BETA AND IL-1 RECEPTOR ANTAGONISTOBTAINED FROM BALF MACROPHAGES IN PATIENTS WITH INTERSTITIAL LUNG-DISEASES

Citation
T. Mikuniya et al., QUANTITATIVE-EVALUATION OF THE IL-1-BETA AND IL-1 RECEPTOR ANTAGONISTOBTAINED FROM BALF MACROPHAGES IN PATIENTS WITH INTERSTITIAL LUNG-DISEASES, Sarcoidosis vasculitis and diffuse lung diseases, 14(1), 1997, pp. 39-45
Citations number
23
Categorie Soggetti
Respiratory System
ISSN journal
11240490
Volume
14
Issue
1
Year of publication
1997
Pages
39 - 45
Database
ISI
SICI code
1124-0490(1997)14:1<39:QOTIAI>2.0.ZU;2-#
Abstract
Our previous reports demonstrated the concomitant release of IL-1 beta and IL-L inhibitory activity in the culture supernatants of BALF macr ophages in both healthy subjects and patients with interstitial lung d iseases. IL-1 inhibitory activities decreased in healthy smokers (HS), and patients with sarcoidosis (Sar), or idiopathic pulmonary fibrosis (IPF), compared with those in healthy nonsmokers (HNS), though an inc rease in IL-1 beta release was not detected. IL-1 inhibitory activity was mainly characterized as IL-1 receptor antagonist (IL-1ra). In this study, we confirmed a decrease in IL-1ra in terms of the amounts of p rotein (enzyme-linked immunoassay) and gene transcripts (reverse trans criptase polymerase chain reaction followed by high performance liquid chromatography). Imbalance between IL-1ra and IL-1 beta was expressed as a molar ratio of IL-1ra/IL-1 beta protein: (Sar; 4.20 +/- 2.06, IP F; 4.26 +/- 3.41, HS; 3.44 +/- 3.09 versus NS 8.33 +/- 2.77: P < 0.001 ). These results were similar in terms of the amounts of gene transcri pts. In conclusion, the imbalance of IL-1 beta and IL-1ra production w as confirmed at three levels: biological activity, amounts of protein, and gene transcript obtained from BALF macrophages in chronic inflamm atory processes in the lungs.