Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography

Authors
Dosio, F Arpicco, S Canevari, S Figini, M Gastaldi, D
Citation
F. Dosio et al., Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography, J CHROMAT A, 830(2), 1999, pp. 329-335
Citations number
22
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A → ACNP
Volume
830
Issue
2
Year of publication
1999
Pages
329 - 335
Database
ISI
SICI code
Abstract
High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chr omatography were compared for their efficiency in purifying immunotoxins (I Ts) containing the ribosome-inactivating protein clavin, which is character ized by a high anionic charge and a low molecular mass. Both methods effici ently removed unreacted clavin from the conjugate crude mixture, but only t he cation-exchange HPMC allowed efficient single-step separation of the unr eacted monoclonal antibody (mAb) from ITs obtained by different coupling pr ocedures. (C) 1999 Elsevier Science B.V. All rights reserved.