A crystal of a typical EF-hand protein grown under microgravity diffracts X-rays beyond 0.9 angstrom resolution

Authors
Declercq, JP Evrard, C Carter, DC Wright, BS Etienne, G Parello, J
Citation
Jp. Declercq et al., A crystal of a typical EF-hand protein grown under microgravity diffracts X-rays beyond 0.9 angstrom resolution, J CRYST GR, 196(2-4), 1999, pp. 595-601
Citations number
20
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF CRYSTAL GROWTH
ISSN journal
00220248 → ACNP
Volume
196
Issue
2-4
Year of publication
1999
Pages
595 - 601
Database
ISI
SICI code
0022-0248(199901)196:2-4<595:ACOATE>2.0.ZU;2-R
Abstract
We report on our recent observation that crystals of a typical EF-hand prot ein (parvalbumin or Pa; Ca-loaded component from pike muscle with isoelectr ic point 4.10) grown under microgravity conditions diffract X-rays to a res olution better than 0.9 Angstrom. The crystals were grown in the US space s huttle and characterized at 100 K, using an X-ray synchrotron beam. An effe ctive atomic resolution has been achieved and substates in the conformation of the protein are observed. Large crystals up to 3 mm were also obtained. (C) 1999 Elsevier Science B.V. All rights reserved.