Knowledge of patterns of organellar inheritance are crucial for analyses of
organellar genetics and population or phylogenetic analyses based on organ
ellar genes. While it has previously been shown that chloroplast DNA is mat
ernally inherited in cotton (Gossypium L.), no studies have assessed the mi
tochondrial genome complement of the allotetraploid species of cotton or ad
dressed inheritance of mitochondrial DNA. In this article we report Souther
n hybridization analyses that indicate that the AD-genome allotetraploid sp
ecies have an A-genome-like mitochondrial genome and provide evidence that
mitochondrial genomes are also maternally inherited in cotton.