MHC class II-dependent NK1.1(+) gamma delta T cells are induced in mice bySalmonella infection

Citation
H. Nishimura et al., MHC class II-dependent NK1.1(+) gamma delta T cells are induced in mice bySalmonella infection, J IMMUNOL, 162(3), 1999, pp. 1573-1581
Citations number
57
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGY
ISSN journal
00221767 → ACNP
Volume
162
Issue
3
Year of publication
1999
Pages
1573 - 1581
Database
ISI
SICI code
0022-1767(19990201)162:3<1573:MCINGD>2.0.ZU;2-M
Abstract
We observed the emergence of a novel population of gamma delta T cells expr essing NK1.1 Ag in the peritoneal cavity of mice infected with Salmonella c holeraesuis. The NK1.1(+) gamma delta T cells accounted for approximately 2 0% of all gamma delta T cells emerging in the peritoneal cavity of C57BL/6 mice and expressed preferentially rearranged V gamma 4-J gamma 1 and V delt a 6.3-D delta 1-D delta 2-J delta 1 genes with N diversity. The gamma delta T cells proliferated vigorously in response to PHA-treated spleen cells an d produced IFN-gamma in the culture supernatant, However, spleen cells from A beta(b)-deficient mice were unable to stimulate the gamma delta T cells. Furthermore, the NK1.1(+)gamma delta T cells were stimulated not only by C hinese hamster ovary (CHO) cells expressing wild-type IA(b) but also by tho se expressing IA(b)/E alpha 52-68 or IA(b)/pigeon cytochrome c-derived anal ogue peptide complex. These proliferation activities were inhibited by mAb specific for IA(b) chain. Consistent,vith these findings, the emergence of NK1.1(+) gamma delta T cells was reduced in the peritoneal cavity of A beta (b)-deficient mice after Salmonella infection, whereas NK1.1(+)gamma delta T cells were rather abundant in the peritoneal cavity of Salmonella-infecte d beta(2)m-deficient mice. Moreover, the NK1.1(+)gamma delta T cells were e asily identified in the thymus of beta(2)m-deficient but not A beta(b)-defi cient mice. Our results indicated that MHC class II expression is essential for development and activation of NK1.1(+)gamma delta T cells in the thymu s and the periphery.