Activated T cells acquire endothelial cell surface determinants during transendothelial migration

Citation
Ri. Brezinschek et al., Activated T cells acquire endothelial cell surface determinants during transendothelial migration, J IMMUNOL, 162(3), 1999, pp. 1677-1684
Citations number
41
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGY
ISSN journal
00221767 → ACNP
Volume
162
Issue
3
Year of publication
1999
Pages
1677 - 1684
Database
ISI
SICI code
0022-1767(19990201)162:3<1677:ATCAEC>2.0.ZU;2-S
Abstract
Activated T cells acquire endothelial cell (EC) plasma membrane constituent s during transendothelial migration. This was assessed using an in vitro mo del system in which human peripheral blood CD4(+) T cells migrated through conflueut monolayers of HUVEC, Flow cytometry of migrated CD4(+) T cells de monstrated that activated, but not resting, T cells acquired a variety of e ndothelial surface determinants, including CD31, CD49d, CD54, CD61, and CD6 2E, The extracellular domains of these molecules were detected on migrated T cells with mAbs, including those directed to the ligand-binding regions. A number of approaches were employed to document that the acquisition of th ese molecules was uniquely accomplished by activated T cells and clearly in volved transfer from both resting and TNF-alpha-activated EC, Acquisition o f endothelial markers by activated T cells occurred as part of the transfer of membrane components, as migrating T cells acquired EC membranes prelabe led with the lipophilic dye, 3'-dihexadecyloxacarbocyanine perchlorate (DiO C-16), along with EC surface proteins. Thus, during transendothelial migrat ion, activated T cells acquire endothelial membrane components, and as a re sult may deliver them to perivascular sites.