Two clinical isolates and the toledo strain of cytomegalovirus contain endothelial cell tropic variants that are not present in the AD169, Towne, or Davis strains

The highly fibroblast-passaged AD169, Towne, and Davis strains of cytomegal ovirus (CMV) were found to have a restricted capacity to infect endothelial cells in vitro. Although such replication could be increased by a combinat ion of low speed centrifugation and sodium butyrate treatment, the extracel lular virus produced was infectious for fibroblasts but not for endothelial cells. In contrast, the low passage Toledo strain, and a low passage fibro blast-grown clinical isolate of CMV, C1F, could be continually passaged in endothelial cells, giving the strains C1FE and Toledo.E. Whilst, using the conditions described above, initial infection of endothelial cells with AD1 69 or C1F resulted in similar titres of extracellular virus as assayed on f ibroblasts, only the virus from the C1F strain was infectious for endotheli al cells. Passage of C1F in fibroblasts decreased its ability to infect end othelial cells, whilst retaining equal ability to infect fibroblasts. Altho ugh endothelial-cell-passaged cell-free C1FE virus was endothelial cell-tro pic, it was still much more infectious for fibroblasts than for endothelial cells. It is concluded that the C1F and Toledo strains, but not the AD169, Towne, or Davis strains, contained endothelial cell tropic variants, which could be lost on passage through fibroblasts, but retained on passage thro ugh endothelial cells. Furthermore, virus in an ex vivo source of CMV, a bl ood specimen, was found to be more tropic for fibroblasts than for endothel ial cells, suggesting that in vivo CMV exists as quasi strains with differe nt cell tropism, some of which might be lost in vitro by passage in an inap propriate cell type. J. Med. Virol. 57:298-307, 1999. (C) 1999 Wiley-Liss, Inc.