A PCR technique for forensic, species-level identification of abalone tissue

Although the incidence of abalone poaching is increasing in South Africa, s everal alleged poachers have been acquitted in cases where the state has be en unable to prove that the confiscated meat is of the local abalone, Halio tis midae. This species is illegally exported to the Far East by poaching s yndicates, a practice that is undermining the legitimate industry. To solve this, a polymerase chain reaction (PCR) technique that targets a portion o f the lysin gene of several abalone species and unequivocally distinguishes between H, midae and H, spadicea (a sympatric congeneric) has been develop ed. The PCR primers specifically amplify approximately 1,300 bp of genomic DNA from dried, cooked, and fresh abalone tissue. A smaller fragment of 146 bp is used for canned abalone. Restriction fragment length polymorphism (R FLP) exploit interspecific polymorphisms that discriminate between these tw o species. The method can also be used to identify H. rubra and can easily be adapted to other abalone species under the same threat of overexploitati on.