O. Ohmori et al., Shifted cytosolic NADP(+)-dependent isocitrate dehydrogenase on 2-D gel inthe brain of genetically epileptic El mice, NEUROCHEM R, 24(3), 1999, pp. 365-369
We observed a spot on two-dimensional (2-D) gel in the epileptic mutant str
ain El mice with a similar molecular weight but with a different isoelectri
c point of approximately 0.2, compared with its mother strain ddY mice. The
collected protein from the El mice was identified as cytosolic NADP(+)-dep
endent isocitrate dehydrogenase by internal amino acid sequencing. The enzy
me is known to be maximally active during the development of the brain and
to play an important role in NADPH production for fatty acids and cholester
ol synthesis. In addition, alterations in cholesterol synthesis early in th
e development of the mammalian brain have been reported to lead to chronic
epilepsy. The results in the present study therefore suggest that cytosolic
NADP(+)-dependent isocitrate dehydrogenase might be involved in the epilep
togenesis of the El mouse.