15-Cis-carotenoids found in the reaction center of a green sulfur bacterium Chlorobium tepidum and in the Photosystem I reaction center of a cyanobacterium Synechococcus vulcanus
Ge. Bialek-bylka et al., 15-Cis-carotenoids found in the reaction center of a green sulfur bacterium Chlorobium tepidum and in the Photosystem I reaction center of a cyanobacterium Synechococcus vulcanus, PHOTOSYN R, 58(2), 1998, pp. 135-142
Carotenoids were extracted, at similar to 4 degrees C in complete darkness
and under nitrogen atmosphere, from the reaction center (RC) of a green-sul
fur bacterium and the Photosystem (PS) I RC of a cyanobacterium; each extra
ct was subjected to high-performance liquid chromatography (HPLC) using an
apparatus equipped with a two-dimensional diode-array detector in order to
spectroscopically identify cis-trans carotenoids while performing HPLC anal
ysis. In the extract from the RC of Chlorobium tepidum, 15-cis and all-tran
s-gamma-carotenes as well as 13-cis-, 15-cis- and all-trans-chlorobactenes
(in the order of elution) were identified, whereas in the extract from the
PS I RC of Synechococcus vulcanus, 15-cis-, all-trans- and 9-cis-beta-carot
enes were found. Thus, the universal presence of 15-cis carotenoids in the
'iron sulfur-type' RCs has been shown in addition to the previous cases of
the 'quinone-type' RCs.