Analyses in transgenic tobacco of the promoter from a Brassica napus gene highly expressed in the stigma

A genomic clone, Pis G363, containing the Brassica napus stigma-expressed g ene Pis 63-2 was isolated and sequenced. The coding region of Pis G363 does not possess introns and shows 82% identity to the nucleotide sequence of a gene from Arabidopsis BAC clone T01B08. A 2-kb promoter fragment from Pis G363 was fused to the coding sequence of the marker enzyme beta-glucuronida se (GUS) and introduced into tobacco via Agrobacterium-mediated transformat ion. The promoter fragment directed expression of the GUS gene in the stigm a of transgenic tobacco. Some transformants also showed relatively low GUS activity in the pollen.