R. Zarate, Tropane alkaloid production by Agrobacterium rhizogenes transformed hairy root cultures of Atropa baetica Willk. (Solanaceae), PL CELL REP, 18(5), 1999, pp. 418-423
Atropa baetica hairy root cultures were induced after infecting stem segmen
ts with Agrobacterium rhizogenes strain ATCC 15834. Accumulation of the tro
pane alkaloids atropine and scopolamine by hairy roots cultured in half- an
d full-strength Murashige and Skoog (MS) medium was high, although this was
; not growth associated. These alkaloids were also released into both liqui
d media. Higher tropane alkaloids present both in hairy roots and liquid me
dium occurred in half MS medium, showing a clear relationship between slow
growth of cultures and higher product accumulation. The pH of both nutrient
media varied as culture progressed, and seemed to be associated with the r
elease of scopolamine. GC-MS analyses showed the presence of a new compound
. namely tigloylpseudotropine: moreover, 3 alpha-isobutyryloxytropane, form
erly found only in plant leaf tissue, was also identified in the hairy root
s.