Micropropagation of Acacia mearnsii from ex vitro material

Multiple shoots were produced from nodal explants, of 30-day-old in vitro g rown seedlings and from pretreated 3- and 9-month-old greenhouse grown Acac ia mearnsii plants, respectively. Explants were sterilized using 0.1% and 0 .2% HgCl2 for 15 min for 3- and 9-month-old explants, respectively. Nodal e xplants were induced to form multiple shoots when placed on MS medium suppl emented with 2.0 mg l(-1) benzyladenine. Rooting of these shoots was achiev ed on MS medium supplemented with 1.0 mg l(-1) indole-3-butyric acid. Plant lets were acclimatized in transparent plastic containers under greenhouse c onditions with a 90% success rate.