Activated charcoal is commonly used in tissue culture media. Its addition t
o culture medium may promote or inhibit in vitro growth, depending on speci
es and tissues used. The effects of activated charcoal may be attributed to
establishing a darkened environment; adsorption of undesirable/inhibitory
substances; adsorption of growth regulators and other organic compounds, or
the release of growth promoting substances present in or adsorbed by activ
ated charcoal.