Disruption and basic phenotypic analysis of six novel genes from the left arm of chromosome XIV of Saccharomyces cerevisiae

We describe here the construction of six deletion mutants and their basic p henotypic analysis in three different backgrounds. The six genes were disru pted in three diploid strains (FY1679, W303 and CEN.PK2) by the long flanki ng homology (LFH) method (Wach, 1996). Transformants were selected as genet icin (G418)-resistant colonies and correct integration of the kanMX4 casset te was checked by colony PCR. Following sporulation of the heterozygous dip loids, tetrads were dissected and scored for segregation of G418-resistance and auxotrophic markers. One of the six ORFs (YNL158w) corresponds to an e ssential gene which has no homology with other genes present in the databas es and has two predicted transmembrane domains. Growth tests performed on d ifferent media at 15 degrees C, 30 degrees C or 37 degrees C with haploid d eletants of the five non-essential genes revealed no apparent phenotype in any of them. Copyright (C) 1999 John Wiley & Sons, Ltd.