B. Djordjevic et al., Clonogenic inactivation of colon cancer-derived cells treated with 5-fluorouracil and indomethacin in hybrid spheroids, ACTA ONCOL, 37(7-8), 1998, pp. 735-739
The clonogenic hybrid spheroid assay has been used to determine the toxicit
y of 5-fluorouracil (5-FU), alone or in combination with indomethacin, in L
oVo cells (a human colon adenocarcinoma line). The principal finding was th
at 5-FU toxicity, determined as loss of colony-forming ability, increased a
s a function of dose (concentration x duration of exposure), and that indom
ethacin causes a generalized alleviation of 5-FU toxicity, but only if give
n concurrently with 5-FU. The implications of these findings in the control
of cancer cells by 5-FU are discussed.