Phage recovery by electroporation of naked DNA into host cells avoids the use of packaging extracts

In this paper, we describe the application of electroporation to deliver ph age DNA into bacterial cells in order to recover it as phage particles. The methodology represents a quicker and cheaper alternative to the use of pac kaging extracts to rescue phage clones stored as naked DNAs. Furthermore, o ur data demonstrate that there were not rearrangements or recombinations be tween phage DNAs when a mixture of different DNAs was electroporated, sugge sting the use of electroporation as a reliable method for construction Of g ene libraries. (C0 1999 Academic Press.