MEASUREMENT OF 2'-DEOXYADENOSINE N1-OXIDE 5'-MONOPHOSPHATE IN MODIFIED DNA, USING CAPILLARY ELECTROPHORESIS

Authors
SIGNORINI N MOLKO D CADET J
Citation
N. Signorini et al., MEASUREMENT OF 2'-DEOXYADENOSINE N1-OXIDE 5'-MONOPHOSPHATE IN MODIFIED DNA, USING CAPILLARY ELECTROPHORESIS, Journal de chimie physique et de physico-chimie biologique, 94(2), 1997, pp. 313-317
Citations number
6
Categorie Soggetti
Biology,"Chemistry Physical
Journal title
Journal de chimie physique et de physico-chimie biologique → ACNP
Volume
94
Issue
2
Year of publication
1997
Pages
313 - 317
Database
ISI
SICI code
Abstract
Adenine N1-oxide is a DNA lesion resulting from indirect effect of sol ar UV radiation through the specific oxidation by hydrogen peroxide un der nonradical conditions. This damage is usually measured using P-32- postlabeling method, which however cannot be used for routine analysis . We propose herein an assay based oil capillary zone electrophoresis which allows a rapid evaluation of the level of modified bases in DNA exposed to oxidative stress.