AFM, a tool for single-molecule experiments

Citation
M. Ludwig et al., AFM, a tool for single-molecule experiments, APPL PHYS A, 68(2), 1999, pp. 173-176
Citations number
16
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science
Journal title
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
ISSN journal
09478396 → ACNP
Volume
68
Issue
2
Year of publication
1999
Pages
173 - 176
Database
ISI
SICI code
0947-8396(199902)68:2<173:AATFSE>2.0.ZU;2-G
Abstract
Force versus elongation curves measured with AFM-related techniques reveal a detailed insight into material properties at the molecular level. Here an overview on different implementations of this technique is given. The late ral distribution of a receptor on a sample surface was determined by mappin g the adhesion force with a corresponding ligand immobilized at the tip, A dextran pattern on a gold surface was measured by mapping the rupture lengt h of the polymer. Stretching single dextran molecules revealed a structural transition in the polysaccharide backbone at forces of 750 pN. The unfoldi ng of individual domains in the modular protein titin was observed and info rmation about the rate dependence of the unfolding forces was obtained.