N. Gorla et al., Application of DNA Fingerprinting to determine paternity in cattle with large congenital abdominal wall defect progeny, ARCH MED V, 30(2), 1998, pp. 151-155
The efficiency of DNA fingerprinting to solve a paternity dispute was evalu
ated in four Hereford bulls of a Cebu/Hereford cattle herd with two calves
affected by a congenital abdominal wall malformation. The test was carried
out using multi-locus probe (CAC)(5). 8 DNA samples -two from the affected
calves, two from their corresponding mothers and 4 from bulls (supposed sir
es)- were processed. These samples were digested with restriction enzyme Ha
e III, blotted onto nylon membranes and hybridised with (CAC)(5). The bands
obtained were visually analysed and data was processed by the computer pro
gram "PATER" (with the "PATER" computer program). The probability of patern
ity (W) was W = 0.80 in one case and W = 0.93 in the other. Despite the kno
wn inbreeding of the bovine breed tested, it was possible to ascertain the
paternity with multilocus DNA fingerprinting (CAC)(5).