Molecular models of acidic peptides from pea bud chromatin and seminal plasma. Divalent cations-mediated interaction with DNA

Citation
L. Mancinelli et al., Molecular models of acidic peptides from pea bud chromatin and seminal plasma. Divalent cations-mediated interaction with DNA, BIOL CHEM, 380(1), 1999, pp. 31-40
Citations number
38
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOLOGICAL CHEMISTRY
ISSN journal
14316730 → ACNP
Volume
380
Issue
1
Year of publication
1999
Pages
31 - 40
Database
ISI
SICI code
1431-6730(199901)380:1<31:MMOAPF>2.0.ZU;2-W
Abstract
Small acidic peptides have been isolated from biological fluids (blood and seminal plasma) and from chromatin of several tissues. Their biological act ivity is related to the control of cell growth and gene expression. This work is an approach to the study of peptide structure-function relatio nship. Purified fractions from seminal plasma and pea bud chromatin were su bjected to fast ion bombardment mass spectrometry. The results obtained wer e analyzed according to biochemical characteristics of the peptides studied and some possible molecular models have been designed. Two of the proposed sequences were synthesized and their biological activity assayed in cells and cell-free systems. The results demonstrate that the synthetic peptides are able to bind to DNA in the presence of divalent cations (Mg2+, Fe2+, Cu 2+) with consequent inhibition of DNA transcription.