Pectin methylesterase gene (pmeA) from Aspergillus oryzae KBN616: Its sequence analysis and overexpression, and characterization of the gene product

Authors
Kitamoto, N Okada, H Yoshino, S Ohmiya, K Tsukagoshi, N
Citation
N. Kitamoto et al., Pectin methylesterase gene (pmeA) from Aspergillus oryzae KBN616: Its sequence analysis and overexpression, and characterization of the gene product, BIOS BIOT B, 63(1), 1999, pp. 120-124
Citations number
23
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
63
Issue
1
Year of publication
1999
Pages
120 - 124
Database
ISI
SICI code
0916-8451(199901)63:1<120:PMG(FA>2.0.ZU;2-Q
Abstract
A gene (pmeA) encoding pectin methylesterase was isolated from a shoyu koji mold, Aspergillus oryzae KBN616, and characterized. The structural gene co mprised 1,370 bp with six introns. The PMEA protein consisted of 331 amino acids with a putative signal peptide of 17 amino acids. The deduced amino a cid sequence was very similar to those of Aspergillus niger PMEA and Asperg illus aculeatus PME1. The pmeA gene was efficiently expressed under control of the A. oryzae TEF1 gene promoter for purification and characterization of the ezymatic properties. PMEA had a molecular mass of 38.5 kDa, a pH opt imum of 5.0, and a temperature optimum of 55 degrees C.