Separation and properties of two acetylacetoin reductases from Bacillus cereus YUF-4

Authors
Hosaka, T Ui, S Mimura, A
Citation
T. Hosaka et al., Separation and properties of two acetylacetoin reductases from Bacillus cereus YUF-4, BIOS BIOT B, 63(1), 1999, pp. 199-201
Citations number
10
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
63
Issue
1
Year of publication
1999
Pages
199 - 201
Database
ISI
SICI code
0916-8451(199901)63:1<199:SAPOTA>2.0.ZU;2-5
Abstract
The separation and purification of two kinds of acetylacetoin reductases (A ACRs) from Bacillus cereus YUF-4 were examined, NADPH-linked AACR (AACR I) and NADH-linked AACR (AACR II) were separated from each other by ammonium s ulfate fractionation, DEAE-cellulose chromatography, and hydroxyapatite chr omatography, The former was purified 3.4-fold with a yield of 10.0%, and th e latter was purified 29-fold with a yield of 15.6%. The two enzymes differ from each other in some enzymic properties such as substrate specificity.