Cloning and sequencing of beta-mannosidase gene from Aspergillus aculeatusno. F-50

The manB gene, coding for a unique beta-mannosidase (MANB) of Aspergillus a culeatus, was cloned from genomic and cDNA libraries, and sequenced. The ge ne consists of 2,811 bp encoding a polypeptide of 937 amino acid residues w ith a molecular mass of 104,214 Da. The A. aculeatus MANB shared amino acid sequence identity with MANB of human (24%), goat (24%), bovine (24%), and Caenorhabditis elegans (22%). When the A. aculeatus MANB was compared with other related enzymes, a Glu residue corresponding to the active site ident ified by the Escherichia coli beta-galactosidase and the human beta-gluclon idase was conserved. This is the first fungal gene that encodes MANB.