ESR study of plasmatic membrane of the transplantable melanoma cells in relation to their biological properties

Using the spin labelling method we studied changes in the structure and dyn amics of molecular mobility in the plasmatic membrane accompanied by a spon taneous alteration of a melanotic melanoma line into an amelanotic form wit h a higher growth rate, changed antigenicity and immunogenicity. The calculated ratio of the low-field line (A) intensity to the central lin e (C) intensity of the spectrum showed statistically significant difference s in the order of parameters in the plasmatic membranes of both forms of me lanocytes. The significantly broader central line (Delta W-0) in the spectra of labell ed amelanotic melanoma cells than in the original cell line indicated chang es in the membrane structure leading to a lowering of the degree of order i n the phospholipid bilayer. It has been suggested that a progression of transplantable melanomas is acc ompanied by an increase in membrane fluidity and reduction in molecular mob ility dynamics within it.