K. Kozlowska et al., ESR study of plasmatic membrane of the transplantable melanoma cells in relation to their biological properties, EXP TOX PAT, 51(1), 1999, pp. 89-92
Using the spin labelling method we studied changes in the structure and dyn
amics of molecular mobility in the plasmatic membrane accompanied by a spon
taneous alteration of a melanotic melanoma line into an amelanotic form wit
h a higher growth rate, changed antigenicity and immunogenicity.
The calculated ratio of the low-field line (A) intensity to the central lin
e (C) intensity of the spectrum showed statistically significant difference
s in the order of parameters in the plasmatic membranes of both forms of me
lanocytes.
The significantly broader central line (Delta W-0) in the spectra of labell
ed amelanotic melanoma cells than in the original cell line indicated chang
es in the membrane structure leading to a lowering of the degree of order i
n the phospholipid bilayer.
It has been suggested that a progression of transplantable melanomas is acc
ompanied by an increase in membrane fluidity and reduction in molecular mob
ility dynamics within it.