S. Ponomarenko et al., Proton gradient-induced changes of the interaction between CF0 and CF1 related to activation of the chloroplast ATP synthase, FEBS LETTER, 443(2), 1999, pp. 136-138
Thylakoid energization by light causes destabilization of CF0CF1 so that th
e peripheral CF1 sector is more readily detached from the membrane by inter
mediate concentrations of the chaotropic salt NaSCN. Here we have investiga
ted the correlation between the proton gradient-induced change of CF0CF1 in
teraction and CF0CF1 activation. The results indicate a close relationship
between the two phenomena. The effect is most probably due to reduction of
the electrostatic interaction between the two subcomplexes CF0 and CF1 as a
consequence of protonations in the interface region. (C) 1999 Federation o
f European Biochemical Societies.