The frequencies of phenotypes, alleles and allelic subtypes of DRB1 and DQB
1 HLA loci in 420 unrelated individuals from the Czech population were dete
rmined. The frequencies of DPB1 alleles of the HLA locus were determined in
92 individuals. The assays were performed using the polymerase chain react
ion (PCR) method or the restriction fragment length polymorphism (RFLP) ana
lysis, The most frequent DRB1 allele was *07, the most frequent DQB1 allele
was *03 and the most frequent DPB1 allele detected was *04. These assays d
efine the extent of polymorphism of the HLA system and are useful for deter
mining the selection strategy of HLA-identical donor-recipient pair suitabl
e for bone marrow transplantation.