S. Kasahara et al., Use of lymphocyte cultures for BrdU replication banding patterns in anuranspecies (Amphibia), GENET MOL B, 21(4), 1998, pp. 471-476
We describe the standardization of lymphocyte culture procedures in order t
o improve cytological preparations of anuran species. This methodology perm
its the use of 5-bromodeoxyuridine (BrdU) treatment to obtain replication b
anding patterns in the chromosomes of these species.