Methane uptake by cultivated Canadian prairie and forest soils

Global methane (CH4) budgets depend on both CH4 sources and sinks. We inves tigated CH4 sink strength of cultivated Canadian prairie and forest soils u sing undisturbed soil columns and an incubation technique in the laboratory . The sink strength was estimated by determining the rate of CH4 uptake by soils with variations in volume of air-filled soil voids, temperature and C H4 concentration. CH4 uptake was attributed to biologically mediated oxidat ion reactions since there was no significant CH4 uptake by autoclaved soils . At field capacity moisture content and 24 degrees C, CH4 uptake under amb ient atmosphere (2.4 mu l CH4 l(-1) air) followed first-order kinetics, tho ugh this was not the case at a lower temperature (4 degrees C). The rate co nstant (h(-1)) of first-order reaction of CH4 uptake at 24 degrees C was 0. 1987 (Orthic Black Chernozemic), 0.0803 (Gray Luvisol, high C), 0.0210 (Gra y Luvisol, low C), and 0.0091 (Saline Black Chernozemic). Relative to the r eaction at 24 degrees C, the percent reduction in amounts of CH4 uptake at 4 degrees C ranged from 49% (Orthic Black Chernozemic) to 80% (Saline Black Chernozemic). increasing the volume of air-filled soil voids increased the rate of CH4 uptake in the Orthic Black Chernozemic and the Gray Luvisol (h igh C) whereas it had little effect on the two other soils. The rate of the reaction was reduced by 30% (Saline Black Chernozemic) to 75% (Gray Luviso l, high C) when the reaction was initiated with higher CH4 concentrations ( 12 and 30 mu l l(-1)). Under these experiment conditions, a two-step proces s of CH4 uptake also was observed, in which the first step was characterize d by a fast CH4 diffusion. These results contribute to the knowledge of rel ative importance of Canadian prairie and forest soils as a CH4 sink and to the significance of local environmental factors in affecting the CH4 sink s trength. (C) 1999 Elsevier Science B.V. All rights reserved.