G. Vanderveen et al., INFLUX OF IMMUNOGLOBULINS FROM THE VASCULAR COMPARTMENT INTO A GRAFTED CORNEA, Investigative ophthalmology & visual science, 38(5), 1997, pp. 876-883
Purpose. To determine the effect of a fresh corneal wound or a healed
corneal scar on the immunodiffusion of immunoglobulins into the cornea
. Methods. F344 rats were immunized with human serum albumin (HSA) 1 w
eek before an autologous rotational keratoplasty of the right cornea o
r 1 year after an autograft was performed. One group of rats also was
treated with gentamicin-dexamethasone ointment in the grafted eye for
1 week after transplantation to reduce the postsurgical inflammatory s
igns, A serum sample was drawn every week and booster injections with
HSA were given after 2 and 3 weeks. At various times after immunizatio
n, groups of rats were killed, blood and aqueous humor samples were ta
ken, and the corneas of both eyes were removed. The corneas were divid
ed into the graft or a 3-mm central button and the peripheral rim and
weighed. The anti-HSA titer was determined in serum, aqueous humor, an
d both parts of the corneas. Results. Up to 5 weeks after transplantat
ion, the grafted corneas contained more anti-HSA immunoglobulins than
did the control eye. One year postgrafting, no difference was seen. In
the first weeks after keratoplasty, influx of anti-HSA from the perip
heral into the central cornea was, however, neither obstructed nor enh
anced. Conclusions. Surgical trauma in itself causes increased influx
of anti-HSA immunoglobulins into the cornea. Within tile cornea, a wou
nd or a scar does not appear to be a barrier for centripetal immunoglo
bulin diffusion.