Control of G(2)/M transition in Xenopus by a member of the p21-activated kinase (PAK) family: A link between protein kinase a and PAK signaling pathways?
S. Faure et al., Control of G(2)/M transition in Xenopus by a member of the p21-activated kinase (PAK) family: A link between protein kinase a and PAK signaling pathways?, J BIOL CHEM, 274(6), 1999, pp. 3573-3579
X-PAKs are involved in negative control of the process of oocyte maturation
in Xenopus (1), In the present study, we define more precisely the events
targetted by the kinase in the inhibition of the G(2)/M transition. We show
that microinjection of recombinant X-PAK1-Cter active kinase into progeste
rone-treated oocytes prevents c-Mos accumulation and activation of both MAP
K and maturation-promoting factor (MPF), In conditions permissive for MAPK
activation, MPF activation still fails. We demonstrate that a constitutive
truncated version of X-PAK1 (X-PAK1-Cter) does not prevent the association
of cyclin B with p34(cdc2) but rather prevents the activation of the inacti
ve complexes present in the oocyte. Proteins participating in the MPF ampli
fication loop, including the Cdc25-activating Polo-like kinase are all bloc
ked. Indeed, using active MPF, the amplification loop is not turned on in t
he presence of X-PAK1. Our results indicate that X-PAK and protein kinase A
targets in the control of oocyte maturation are similar and furthermore th
at this negative regulation is not restricted to meiosis, because we demons
trate that G(2)/M progression is also prevented in Xenopus cycling extracts
in the presence of active X-PAK1.