Nm. Kudoyarova-zubavichene et al., Preparation and use of hyperimmune serum for prophylaxis and therapy of Ebola virus infections, J INFEC DIS, 179, 1999, pp. S218-S223
To obtain hyperimmune serum appropriate for the treatment of filovirus infe
ction, methods were developed to immunize nonsusceptible animals with live
Ebola (EBO) virus preparations, Immune plasma with high ELISA and neutraliz
ation-specific antibody titers was obtained by multiple immunization of she
ep and goats with preparations of Live EBO virus. Goat immunoglobulin was p
repared by Cohn's method and tested on guinea pigs, using an EBO virus stra
in that is highly pathogenic for guinea pigs. Prophylaxis with these immuno
globulins within 48 h after infection was effective in challenge experiment
s, with a log(10) prophylaxis index as high as 1.92 +/- 0.52. Other studies
have shown that equine anti-EBO virus immunoglobulins worked well in baboo
ns. The goat immunoglobulins were also tested in preclinical trials on labo
ratory animals; after being positively evaluated, they were administered to
volunteers in clinical trials for biologic safety and reactivity, and they
were administered to researchers suspected of becoming infected with EBO d
uring their experimental work. These immunoglobulins may be useful for the
emergency treatment of persons accidentally infected with EBO.