Choroidal tuberculosis diagnosed by polymerase chain reaction - A clinicopathologic case report

Purpose: To show the use of the polymerase chain reaction (PCR) in a granul omatous choroidal lesion to support a diagnosis of tuberculosis. Design: Testing: Observational case report. Testing: Nucleic acid target amplification of a choroidal specimen using PC R for detection of Mycobacterium tuberculosis was tested. Main Outcome Measures: Positive nucleic acid target amplification for M. tu berculosis in the ocular sample was measured. Results: PCR was positive for M. tuberculosis with appropriate negative con trols. Conclusions: PCR was thought to be a useful supportive technique in the dia gnosis of choroidal tuberculosis.