OXYTOCIN RECEPTOR-MEDIATED ACTIVATION OF PHOSPHOINOSITIDASE-C AND ELEVATION OF CYTOSOLIC CALCIUM IN THE GONADOTROPE-DERIVED ALPHA-T3-1 CELL-LINE

Citation
Jj. Evans et al., OXYTOCIN RECEPTOR-MEDIATED ACTIVATION OF PHOSPHOINOSITIDASE-C AND ELEVATION OF CYTOSOLIC CALCIUM IN THE GONADOTROPE-DERIVED ALPHA-T3-1 CELL-LINE, Endocrinology, 138(5), 1997, pp. 2049-2055
Citations number
36
Categorie Soggetti
Endocrynology & Metabolism
Journal title
ISSN journal
00137227
Volume
138
Issue
5
Year of publication
1997
Pages
2049 - 2055
Database
ISI
SICI code
0013-7227(1997)138:5<2049:ORAOPA>2.0.ZU;2-D
Abstract
Gonadotropes synthesize and secrete LH and FSH under the control of Gn RH, which acts via phosphoinositidase G (PIC)-linked G protein coupled receptors. Additionally, gonadotropin released from the pituitary is influenced by oxytocin, a peptide that has been shown to play a role i n generation of the preovulatory LH surge. Although oxytocin receptors are present in the pituitary, studies have identified their presence on lactotropes but not on gonadotropes, raising the question of which cells act as the direct target of oxytocin in gonadotrope regulation. In this study, we examined effects of oxytocin on alpha T3-1 cells, a gonadotrope-derived cell line. Oxytocin, vasopressin, and vasotocin ea ch stimulated accumulation of [H-3]inositol phosphates in cells prelab eled with [H-3]inositol, indicating activation of PIG. The rank order of potency (oxytocin > vasotocin > vasopressin) and sensitivity to inh ibition by oxytocin and vasopressin receptor antagonists, revealed the effect to be mediated by oxytocin-selective receptors. Like other PIC activators, these nonapeptides caused biphasic (spike-plateau) increa ses in the cytosolic Ca2+. The spike response to oxytocin and GnRH wer e both retained in Ca2+-free medium, reflecting mobilization of intrac ellular Ca2+, and were comparably reduced by thapsigargin, implying mo bilization of Ca2+ from a shared thapsigargin-sensitive intracellular pool. Brief stimulation with oxytocin, vasopressin, or vasotocin preve nted subsequent Ca2+ responses to oxytocin, but not to GnRH, suggestin g that the oxytocin receptor undergoes rapid homologous desensitizatio n and reinforcing the interpretation that the nonapeptides act via the same receptor type. Oxytocin did not increase Ca2+ in cells stimulate d with Gr;RK, whereas GnRH caused a spike Ca2+ increase even in the pr esence of oxytocin, implying that different mechanisms of desensitizat ion (Ca2+ pool depletion and receptor uncoupling) are operating for tw o distinct PIG-coupled receptors in these cells. The demonstration tie r, that oxytocin acts directly via PIG-linked, oxytocin-selective rece ptors to increase cytosolic Ca2+ in a gonadotrope-derived cell line is consistent with the possibility that oxytocin has a comparable effect on nonimmortalized gonadotropes.