M. Scott et al., Crystallization and preliminary X-ray analysis of arabinanase A from Pseudomonas fluorescens subspecies cellulosa, ACT CRYST D, 55, 1999, pp. 544-546
Crystals of 1,5-alpha-arabinanase A from Pseudomonas fluorescens subspecies
cellulosa have been obtained by vapour diffusion. The crystals belong to t
he space group P6(1)22 with unit-cell parameters a = b = 91.6, c = 179.4 An
gstrom with one molecule in the asymmetric unit. The native crystals and, t
o a much greater extent, heavy-atom soaked crystals are sensitive to radiat
ion which necessitates cryocooling. Suitable cryocooling conditions have be
en established, though a shrinkage of the unit cell is observed, with a = b
= 88.8 and c = 176.9 Angstrom.