Crystallization of L-aspartate oxidase, the first enzyme in the bacterial de novo biosynthesis of NAD

Authors
Bacchella, L Lina, C Todone, F Negri, A Tedeschi, G Ronchi, S Mattevi, A
Citation
L. Bacchella et al., Crystallization of L-aspartate oxidase, the first enzyme in the bacterial de novo biosynthesis of NAD, ACT CRYST D, 55, 1999, pp. 549-551
Citations number
18
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
55
Year of publication
1999
Part
2
Pages
549 - 551
Database
ISI
SICI code
0907-4449(199902)55:<549:COLOTF>2.0.ZU;2-P
Abstract
The flavoenzyme L-aspartate oxidase from Escherichia coli was crystallized using the hanging-drop vapour-diffusion technique with PEG 4000 as precipit ant. The crystals belong to space group P3(1)21 (or P3(2)21) with unit-cell parameters a = b = 84.9, c = 159.9 Angstrom. A solvent content of 42% corr esponds to a monomer (60 kDa) in the asymmetric unit. A complete 2.8 Angstr om resolution data set was collected using a rotating-anode X-ray generator .