P. Sabatino et al., Crystallization and preliminary X-ray study of chloroplast glyceraldehyde-3-phosphate dehydrogenase, ACT CRYST D, 55, 1999, pp. 566-567
Glyceraldehyde-3-phosphate dehydrogenase from spinach chloroplasts has been
crystallized by vapour diffusion in the pH range 7-8.5 in (NH4)(2)SO4 and
Tris-HCl buffer or potassium phosphate buffer at room temperature. Crystals
of the A(4) isoform, grown at pH 8.5 in Tris-HCl buffer, diffract to 3.0 A
ngstrom (at 100 K) using synchrotron radiation. The crystals belong to the
orthorhombic C222 space group, with unit-cell dimensions a = 145.9, b = 185
.9 and c = 106.3 Angstrom, and probably contain one tetramer per asymmetric
unit. Structure determination by molecular replacement is in progress.