Preliminary X-ray analysis of a new crystal form of recombinant pig kidneyDOPA decarboxylase

Citation
Vn. Malashkevich et al., Preliminary X-ray analysis of a new crystal form of recombinant pig kidneyDOPA decarboxylase, ACT CRYST D, 55, 1999, pp. 568-570
Citations number
18
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
55
Year of publication
1999
Part
2
Pages
568 - 570
Database
ISI
SICI code
0907-4449(199902)55:<568:PXAOAN>2.0.ZU;2-N
Abstract
DOPA decarboxylase is responsible for the synthesis of the key neurotransmi tters dopamine and serotonin via decarboxylation of L-3,4-dihydroxyphenylal anine (L-DOPA) and L-5-hydroxytryptophan, respectively. The crystals of rec ombinant DOPA decarboxylase differ from those previously reported for the e nzyme purified from pig kidney. They belong to space group P622 with unit-c ell dimensions a = b = 302.6, c = 178.1 Angstrom. Both the self-rotation fu nction and the good diffraction quality of these crystals (2.5 Angstrom on a synchrotron source) suggest that there should be at least three protein d imers in the asymmetric unit. Diffraction data sets have been collected for the native enzyme and a heavy-atom derivative.