Tj. Li et al., Effects of hypoxia/reoxygenation and cytokines on adhesion of leukocytes to cerebral microvascular endothelial cells, ACT PHAR SI, 20(2), 1999, pp. 137-140
AIM: To study the influence of hypoxia/reoxygenation ( WR) on the adhesion
of neutrophils (Neu) and monocytes (Mon) to cultured bovine cerebral microv
ascular cells induced by tumor necrosis factor alpha (TNF-alpha) or interle
ukin 1-alpha (IL-1 alpha). METHODS: Bovine cerebral microvascular endotheli
al cells (CMEC) and smooth muscle cells (CMSMC) were exposed to normoxia or
WR in the presence or absence of TNF-alpha (IL-1 alpha). The number of adh
esions of Mon and Neu to CMEC and CMSMC was measured by flow cytometry. RES
ULTS: CMEC were subjected to hypoxia for 2 h followed by reoxygenation with
TNF-alpha (2 mu g.L-1) for 2 h, the adhesion rate of CMEC for Mon and Neu
were increased to 35.0 % +/- 0.9 % and 36.0 % +/- 0.6 % (the TNF-alpha trea
ted group was 28.9 % +/- 1.1 % and 28.8 % +/- 1.3 %), respectively. CMSMC w
ere treated as above, the adhesion rate of Mon and Neu was enhanced to 49.9
% +/- 0.4 % and 43.9 % +/- 1.4 % (the TNF-alpha treated group was 34.0 % /- 1.9 % and 34.0 % +/- 1.3 %), respectively. CMEC and CMSMC were treated b
y IL-lcr, similar results were obtained. CONCLUSION: WR enhanced the adhesi
on of Mon and Neu to CMEC and CMSMC induced by TNF-alpha or IL-1 alpha.