Balance between proinflammatory cytokines and their inhibitors in bronchial lavage from patients with status asthmaticus

Status asthmaticus (SA) is an acute respiratory failure combining an acute bronchospastic reaction with a severe airway inflammation. We previously re ported an important influx of neutrophils and an increased secretion of int erleukin-8 (IL-8) in patients with SA. The aim of this prospective study wa s to evaluate in bronchial lavage (BL) of patients with SA (n = 9) under me chanical ventilation (MV) the concentrations of cytokines and related media tors which have the ability to modulate inflammation, either proinflammator y (interleukin-1 beta [IL-1 beta], IL-6, tumor necrosis factor-alpha [TNF-a lpha]), or antiinflammatory mediators (IL-10, transforming growth factor-be ta 1 [TGF-beta 1]), interleukin-1 receptor antagonist [IL-1Ra], soluble TNF receptor I and II [sTNFRI and II]. To determine the relative importance of both pro- and anti-inflammatory mediators, the net inflammatory activity w as analyzed by the capacity of BL fluids (BLF) to increase intercellular ad hesion molecule-1 (ICAM-1) expression in the human lung A549 epithelial cel l line. These data were compared with those obtained from patients who requ ired MV without respiratory disease (V, n = 4), controlled asthma (A, n = 1 1), and nonsmoking healthy volunteers (C, n = 8). Levels of IL-l, IL-6, TNF -alpha, and of the active form of TCF-beta 1 were significantly higher in S A compared with the other groups. The concentrations of IL-1Ra, IL-10, the latent form of TCF-beta 1, and of the sTNFRI and II were not significantly different between SA and V, albeit higher in SA than in A and C. The ratio between IL-l Ra and IL-1 beta was significantly higher in patients with SA compared with the other groups, whereas there was no difference for the rat io between both types of sTNFR and TNF-alpha. Despite a marked increase of anti-inflammatory mediators in BL from patients with SA, the net inflammato ry activity was found to be proinflammatory and mainly due to the presence of bioactive IL-1 beta (79% inhibition of ICAM-1 expression with anti-IL-1 beta antibodies) and to a lesser extent TNF-alpha (32% inhibition with anti -TNF-alpha antibodies).