Pesticide determination by stopped-flow fluoroimmunoassay using Cresyl Violet as label

Citation
Mp. Aguilar-caballos et al., Pesticide determination by stopped-flow fluoroimmunoassay using Cresyl Violet as label, ANALYT CHIM, 381(2-3), 1999, pp. 147-154
Citations number
19
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICA CHIMICA ACTA
ISSN journal
00032670 → ACNP
Volume
381
Issue
2-3
Year of publication
1999
Pages
147 - 154
Database
ISI
SICI code
0003-2670(19990216)381:2-3<147:PDBSFU>2.0.ZU;2-Y
Abstract
A homogeneous stopped-flow fluoroimmunoassay method based on the use of Cre syl Violet as label is described for the first time and applied to the dete rmination of the pesticide 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). The tracer showed an increase in its fluorescence intensity when reacted with the antibody, so that the initial rate of this reaction and the equilibrium signal were used as the analytical parameters, which were measured in 2 an d 50 s, respectively. The dynamic range of the calibration graph obtained f or the proposed kinetic and equilibrium methods were 0.02-10 ng ml(-1) and 0.1-100 ng ml(-1) respectively, and the detection limits achieved were 6 an d 30 pg ml(-1), respectively. The relative standard deviation was close to 3%. The recoveries obtained by applying the method to several spiked liquid food samples (spring water, white wine and grape juice) ranged from 94.4% to 105.6%. (C) 1999 Elsevier Science B.V. All rights reserved.