Time-resolved fluorometric detection of DNA using a tetradentate beta-diketonate europium chelate as a label

Authors
Yoshikawa, K Yuan, JL Matsumoto, K Kimura, H
Citation
K. Yoshikawa et al., Time-resolved fluorometric detection of DNA using a tetradentate beta-diketonate europium chelate as a label, ANAL SCI, 15(2), 1999, pp. 121-124
Citations number
26
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL SCIENCES
ISSN journal
09106340 → ACNP
Volume
15
Issue
2
Year of publication
1999
Pages
121 - 124
Database
ISI
SICI code
0910-6340(199902)15:2<121:TFDODU>2.0.ZU;2-O
Abstract
A tetradentate beta-diketonate europium chelate, 4,4'-bis(1 ",1 ", 1 ",2 ", 2 ",3 ",3 "-heptafluoro-4 ",6 "-hexanedion-6 "-yl)-chloro- sulfo-o-terpheny l(BHHCT)-Eu3+, was labeled to a streptavidin-bovine serum albumin conjugate for time-resolved fluorometric detection of lambda DNA by hybridization wi th a biotinylated probe DNA in a microtiter well. The method gave a detecti on limit of 23 pg/well (solid-phase measurement) and 1 pg/well (solution-ph ase measurement) for specific DNA.