Potentiometric determination of NAD(P)H coenzymes using a nitrite-selective membrane electrode and a nitrate reductase enzyme and the application to glucose assay in human serum
T. Katsu et al., Potentiometric determination of NAD(P)H coenzymes using a nitrite-selective membrane electrode and a nitrate reductase enzyme and the application to glucose assay in human serum, ANAL SCI, 15(2), 1999, pp. 135-139
NAD(P)H coenzyme concentrations were determined with the combination of a n
itrite-selective membrane electrode and a nitrate reductase enzyme. The met
hod was based on the detection of nitrite formed by the enzymatic reaction
of NAD(P)H with nitrate catalyzed by nitrate reductase. The lower detection
limits for both these coenzymes were around 100 mu M. This system was appl
ied to detect glucose by combining an additional glucose dehydrogenase enzy
me. Serum glucose levels could be determined through the detection of nitri
te.