Detection of shiga-like toxin (stx(1) and stx(2)), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR

Authors
Fagan, PK Hornitzky, MA Bettelheim, KA Djordjevic, SP
Citation
Pk. Fagan et al., Detection of shiga-like toxin (stx(1) and stx(2)), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR, APPL ENVIR, 65(2), 1999, pp. 868-872
Citations number
32
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
65
Issue
2
Year of publication
1999
Pages
868 - 872
Database
ISI
SICI code
0099-2240(199902)65:2<868:DOST(A>2.0.ZU;2-L
Abstract
A multiplex PCR was developed for the rapid detection of genes encoding Shi ga toxins 1 and 2 (stx(1) and stx(2)), intimin (eaeA), and enterohemolysin A (hlyA) in 444 fecal samples derived from healthy and clinically affected cattle, sheep, pigs, and goats. The method involved non-solvent-based extra ction of nucleic acid from an aliquot of an overnight culture of feces in E C (modified) broth. The detection limit of the assay for both fecal samples and pure cultures was between 18 and 37 genome equivalents. stx(1) and hly A were the most commonly encountered virulence factors.