Aminoglycoside antibiotics - Two decades of their HPLC bioanalysis

Several reviews have been published on high-performance liquid chromatograp hic (HPLC) methods for the determination of aminoglycoside antibiotics (ami noglycosides) in biological fluids [e.g. Nilsson-Ehle, I. (1983). J. Liq. C hromat. 6: 251]. Of these, the paper by Maitra et al. [(1979a). Clin. Chem. 25: 1361.] briefly summarizes the early 2-3 years of experience on HPLC as saying of amikacin, gentamicin, netilmicin and tobramycin in body fluids. T he reviews by Nilsson-Ehle, I. [(1983). J. Liq. Chromat. 6: 251] and by Min er, D. J. [(1985). Antibiotics. In Therapeutic Drug Monitoring and Toxicolo gy by Liquid Chromatography, (Wong S. H. Y., ed.), ch.10, p. 269. Marcel De kker, New York and Basel.] devoted to the monitoring of antibiotics, also e valuated the first 6-8 years of the application of HPLC assays for the amin oglycosides amikacin, gentamicin, netilmicin, sisomicin and tobramycin. Thi s report presents a great majority of the HPLC assay methods published duri ng the last two decades for determining practically a dozen different amino glycoside antibiotics in body fluids, particularly in the serum or plasma, and in urine. Copyright (C) 1999 John Wiley & Sons, Ltd.