P. Prados et al., Changes of plasma L-arginine levels in spontaneously hypertensive rats under induced hypotension, BIOMED CHRO, 13(1), 1999, pp. 27-32
Nicardipine, a dihydropyridine type calcium channel blocker, was infused at
two flow-rates into spontaneously hypertensive (SH) and control normotensi
ve Wistar-Kyoto (WKY) rats (young, 6-week-old and adult, 23-week-old, n = 5
) under pentobarbital anesthesia, to cause hypotension. Mean arterial blood
pressure and the concentrations of plasma amino acids and norepinephrine (
NE) were measured before infusion and at each step of the infusion. The red
uction in blood pressure caused by nicardipine induced a decrease in plasma
L-arginine concentration in both young and adult SH rats, this effect bein
g larger in adult rats. There was no significant change in plasma levels of
L-arginine in age-matched WKY rats. The concentration of other amino acids
did not change in both rat strains. On the contrary, there was an increase
in plasma NE concentration in both SH and WKY rats after infusion with nic
ardipine. Plasma L-arginine concentration showed a good inverse correlation
with the logarithm of plasma NE concentration in SH and WKY rats and the c
orrelation was expressed as Y = -alpha log(X) + m (Y, plasma L-arginine con
centration (nmol/mL); X, plasma NE concentration (pmol/mL); alpha, a slope;
and m, an intercept). alpha, 43.0 and 4.35 for 23-week-old SH and WKY rats
, respectively, and 17.0 and 4.0 for 6-week-old SH and WKY rats, respective
ly. The present data together with previous data suggest a direct noradrene
rgic stimulation of the synthesis of nitric oxide (NO) from L-arginine. The
findings also indicate an impairment of the L-arginine metabolism or pools
in SH rats compared with WKY rats. The deficiency of L-arginine increases
with the age of SH rats and could be related to the development and mainten
ance of hypertension due to inefficient production of NO. Copyright (C) 199
9 John Wiley & Sons, Ltd.