A kinetic study of ornithine carbamyl transferase (OCT) from Streptococcus
faecalis has been carried out, employing a new HPLC method based on the dir
ect determination of citrulline. A different kinetic pattern was observed i
n tris(hydroxymethyl)-aminomethane (TRIS) and triethanolamine (TEA) buffers
: a ping-pong or an ordered sequential mechanism are suggested, respectivel
y. The inhibition of Tris buffer on OCT reaction has also been demonstrated
. The inhibition of Tris buffer on OCT reaction has also been demonstrated.
Copyright (C) 1999 John Wiley & Sons, Ltd.