Buffer effect on the kinetics of ornithine carbamyl transferase by HPLC

Authors
Carunchio, V Girelli, AM Messina, A
Citation
V. Carunchio et al., Buffer effect on the kinetics of ornithine carbamyl transferase by HPLC, BIOMED CHRO, 13(1), 1999, pp. 65-69
Citations number
18
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOMEDICAL CHROMATOGRAPHY
ISSN journal
02693879 → ACNP
Volume
13
Issue
1
Year of publication
1999
Pages
65 - 69
Database
ISI
SICI code
0269-3879(199902)13:1<65:BEOTKO>2.0.ZU;2-1
Abstract
A kinetic study of ornithine carbamyl transferase (OCT) from Streptococcus faecalis has been carried out, employing a new HPLC method based on the dir ect determination of citrulline. A different kinetic pattern was observed i n tris(hydroxymethyl)-aminomethane (TRIS) and triethanolamine (TEA) buffers : a ping-pong or an ordered sequential mechanism are suggested, respectivel y. The inhibition of Tris buffer on OCT reaction has also been demonstrated . The inhibition of Tris buffer on OCT reaction has also been demonstrated. Copyright (C) 1999 John Wiley & Sons, Ltd.