Cytogenetic profile of lymphoma of follicle mantle lineage: Correlation with clinicobiologic features

Conventional chromosome analysis (CCA) and interphase fluorescence in situ hybridization (FISH) was performed in 42 patients with mantle-cell lymphoma (MCL), with BCL1 rearrangement. The t(11;14)(q13;q32) or 11q abnormalities were detected by CCA in 34 cases, 20 of which had additional aberrations. A normal karyotype was observed in 8 cases. Probes detecting the chromosome aberrations that: were observed in at least: 3 cases by CCA, ie, +12, 13q1 4 deletion, and 17p deletion, were used for interphase FISH analysis. FISH detected total or partial +12, 13q14 deletion and 17p- in 28.5%, 52.4%, and 26% of the cases, respectively. The presence of these anomalies was not a function of karyotype complexity. Based on the results of CCA/FISH, three g roups of increasing karyotype complexity were recognized: group 1, includin g 11 patients without detectable aberrations in addition to BCL1 rearrangem ent; group 2, including 14 patients with 1 to 2 additional anomalies; and g roup 3, including 17 patients with three or more additional anomalies. Clin ical parameters associated with shorter survival were male sex (P = .006) a nd primary lymph-node involvement compared with primary bone marrow involve ment (P = .015). Trisomy 12 was the only single cytogenetic parameter predi ctive of a poor prognosis (P = .006) and the best prognostic indicator was the derived measure of karyotype complexity (P < .0001), which maintained s tatistical significance in multivariate analysis (P<.0001). We arrived at t he following conclusions: 13q14 deletion occurs at a high Incidence in MCL; 17p deletion and total/partial +12 are relatively frequent events in MCL, the latter aberration being associated with a shorter survival; and the deg ree of karyotype complexity has a strong impact on prognosis in this neopla sia. (C) 1999 by The American Society of Hematology.